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KEGG pathway: DNA replication

Pathway hsa03030
Name DNA replication
Members PCNA MCM7 MCM5 POLE2 MCM3 MCM2 RNASEH2A RFC2 RFC4 MCM6 POLA1 POLD2 POLA2 RPA1 PRIM1 POLE MCM4 POLE3 RPA3 RFC5 POLD3 RFC1 RNASEH2C SSBP1 LIG1 POLD1 POLD4 FEN1 RFC3 RPA2 DNA2 RNASEH1 RPA4 POLE4 RNASEH2B
Description A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.